Effects of the Shenxiong Injection on the inflammatory factors changes after focal cerebral ischemia-reperfusion in rats
- VernacularTitle:参芎注射液对脑缺血再灌注大鼠炎症因子变化的影响
- Author:
Xiaodong CHEN
;
Chunfeng LIU
;
Yongjun CAO
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia/reperfusion;
Inflammation;
Shenxiong Injection;
Rats
- From:
Chinese Journal of Practical Internal Medicine
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of NF-?B,TNF-?,ICAM-1 in rat's brain tissue and the sequential changes of serum IL-1?,IL-6,IL-10 after focal cerebral ischemia-reperfusion,and to observe the influence of the Shenxiong Injection.Methods Fifty-four male rats were randomly divided into control,Ligustrazin-treated and Shenxiong-treated group.Rats models of focal cerebral ischemia-reperfusion(I/R)were made by using suture.Drew back sutures 1 hour after the blood supply were blocked.A series of brain and blood samples were obtained directly 6,12 and 24 hours respectively after reperfusion in three groups.The expression of NF-?B,TNF-? and ICAM-1 in the brain tissue was determined by immuno-histochemical method.Serum levels of IL-1?,IL-6 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA).Results As time of I/R prolonged,the expression of TNF-?,ICAM-1 upregulated,but NF-?B reached the maximum at R12 h in three groups.Compared with control group,expression of NF-?B,TNF-?,ICAM-1 in Shenxiong-treated group decreased significantly(P
