Tracing transmembrane transport of amino-glucomannan
- VernacularTitle:荧光胺示踪氨基葡甘露聚糖的跨细胞膜能力与转运机制
- Author:
Yuan GUO
;
Yongkang LIU
;
Ying DU
;
Hongtao LI
;
Guomin CHEN
- Publication Type:Journal Article
- Keywords:
amino-glucomannan;
fluram;
fluram labeling;
transmembrane transport
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To label the amino-glucomannan(AGM) with fluram and to explore the properties of the compound of fluram-AGM in transmembrane transport.Methods After AGM was labeled with fluram,the peripheral blood mononuclear cells(PBMC) and HepG2 were respectively cultured with Fluram-AGM,then observed under fluorescence microscope with violet light as exciting light.Results Both PBMC and HepG2 showed intracellular indigotic fluorescence.Conclusion AGM can be transported into cells across cell membrane.
