Effects of adenovirus vector mediated Cx43 gene modification on GJIC function of acute leukemia bone marrow stromal cells
- VernacularTitle:腺病毒介导Cx43基因修饰对急性白血病骨髓基质细胞GJIC功能的影响
- Author:
Yingjian SI
;
Xi ZHANG
;
Yao LIU
;
Yanmin TONG
;
Xinghua CHEN
;
Li GAO
;
Lei GAO
;
Xiangui PENG
;
Lixia GUANG
;
Yuqing WANG
- Publication Type:Journal Article
- Keywords:
Cx43;
GJIC;
recombinant adenovirus;
acute leukemia bone marrow stromal cells;
GFP
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene expression of connexin43(Cx43) and its effect on gap junction intercellular communication(GJIC) of acute leukemia bone marrow stromal cells(ALBMSCs).Methods After ALBMSCs were transfected by recombinant adenovirus Ad-Cx43-GFP,the expression of report gene GFP and the transfection efficiency were monitored by fluorescent microscopy.RT-PCR,Western blot and immunocytochemical method were used to detect the mRNA and protein expressions of Cx43.Dye transfer procedure was performed to examine the GJIC function.Results After transfected by Ad-Cx43-GFP for 24 h,the expression of GFP in ALBMSCs was detected by fluorescent microscopy and the transfection efficiency was(82.7?2.16)%;The mRNA and protein expressions of Cx43 in ALBMSCs transfected by Ad-Cx43-GFP were higher than those not transfected by Ad-Cx43-GFP(P
