Restrain effects of Nordy on tubule-like structure formation of endothelial cells in a three-dimensional culture
- VernacularTitle:诺帝对体外内皮细胞三维成型的抑制作用
- Author:
Jiasi BAI
;
Jingquan SHI
;
Jiang ZHENG
;
Xiuwu BIAN
- Publication Type:Journal Article
- Keywords:
angiogenesis;
glioma;
endothelial cell;
cell cycle;
apoptosis
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the new drug Nordy on malignant glioma cells induced tubule-like structure formation of endothelial cells in a three dimensional culture system with collagen coating.Methods The endothelial cells were divided into three groups.Group A were cultured on the DMEM media coated with collagen;group B were cultured in 2:1(V/V) DMEM to the supernatant of malignant glioma cell culture;group C,based on the group B,were added with Nordy of different concentrations.The cell cycle and apoptosis of ECV304 cells were determined and the process of tubular formation were observed.Moreover,the parameters such as the forming curves,outer and inner diameter,wall thickness and length of the tubules were measured and compared between groups B and C.Results The cell cycle of endothelial cells were restrained,and some cells were induced to apoptosis.The tubule-like structure formation was inhibited,and the tubules in group B appeared earlier,larger in diameter and longer in length than group C.Conclusion The tubular formation induced by malignant glioma cells could be restrained by the new drug Nordy.
