Effect of different types of flavonoids on H_2O_2-induced apoptosis in neonatal rat cardiomyocytes
- VernacularTitle:不同类型黄酮对过氧化氢诱导的心肌细胞凋亡的影响
- Author:
Jingkun LU
;
Liwei WANG
;
Fengzhi LIU
- Publication Type:Journal Article
- Keywords:
flavonoids;
apoptosis of cardiomyocytes;
antioxidant
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of different types of flavonoids, galangin (flavonol), hesperetin (flavonone) and hydroxysafflor yellow A (HYSA, chalcone), on cardiomyocytic injury induced by H2O2, and explore the possible signal pathways involved. METHODS: The cytotoxicity of different flavonoids was determined by MTT assay. Apoptosis rate was determined by flow cytometry (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Immunohistochemistry was used for detection of Bcl-2, Bax and Caspase-3 protein. RESULTS: It showed that each flavonoid did not have noticeable cytotoxicity at a concentration of 5 ?mol/L by MTT assay. Flavonoids at concentrations of 5, 15 and 30 ?mol/L significantly increased cell viability compared to model group induced by H2O2 (100 ?mol/L). Flavonoids also increased apoptosis rate and neorobiosis rate determined by FCM compared to model group. Galangin and hesperetin significantly decreased the apoptotic rate determined by TUNEL and the expression of Caspase-3 and increased the ratio of Bcl-2/Bax (P
