Effects of resveratrol on the expression of FAK and the level of phosphorylated FAK in highly metastatic ovarian carcinoma HO-8910PM cell line
- VernacularTitle:白藜芦醇对高转移卵巢癌细胞中FAK表达及磷酸化水平的影响
- Author:
Yanmei QIN
;
Ke LI
;
Taiping HE
;
Lier MO
;
Nianci LIANG
- Publication Type:Journal Article
- Keywords:
resveratrol;
FAK;
expression;
ovarian carcinoma
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of resveratrol on the expression of FAK and the level of phosphorylated FAK, and to investigate its possible mechanism of resveratrol on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells line. Methods MTT assay was used to examine cytotoxicity of resveratrol on HO-8910PM cells after 24 h treatment; The expression of FAK protein and the level of phosphorylated FAK were assessed by Western blotting analysis. Results MTT assay showed that resveratrol had weak cytotoxicity on HO-8910PM cells after 24 h treatment, its IC50 value was 163.40?2.48 ?mol?L-1. The expression of FAK and the level of phosphorylated FAK decreased obviously in HO-8910PM cells treated with 25~100 ?mol?L-1 resveratrol for 24 h. Conclusion The mechanism of resveratrol inhibiting the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells line involves the expression of FAK and the level of phosphorylated FAK.
