Impact of hydrogen sulfide donor on the content and metabolism of collagen in rats with pulmonary hypertension induced by high pulmonary blood flow
- VernacularTitle:硫化氢供体对高肺血流性肺动脉高压大鼠肺动脉胶原含量及代谢的影响
- Author:
Xiaohui LI
;
Junbao DU
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
high pulmonary blood flow;
pulmonary hypertension;
collagen
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim The study was designed to explore the possible impact of sodium hydrosulfide NaHS on the content and metabolism of collagen in rats with pulmonary hypertension induced by high pulmonary blood flow. Methods Thirty-two male SD rats, weighing 140~160 g, were randomly divided into 4 groups, shunt group (n=8), shunt+NaHS group (n=8), sham group (n=8) and sham+NaHS group (n=8). Rats in shunt group and shunt+NaHS group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary flow. In the sham group and sham+NaHS group, rats experienced the same experimental processes except the shunting procedure. After 11 weeks of experiment, systolic pulmonary artery pressure (SPAP) of rats was detected using a right cardiac catheterization procedure. Lung tissue hydrogen sulfide(H2S) content of rats was determined by a modified sulfide electrode method. Pulmonary artery collagen Ⅰ,collagen Ⅲ,MMP-13 and TIMP-1 protein expression of rats was investigated by immunohistochemistry.Results After 11 weeks of experiment, SPAP increased significantly, whereas lung tissue hydrogen sulfide(H2S) content decreased significantly in rats of shunt group as compared with those of sham group (P
