Inhibitory effects of Zedoray Rhizome Abstracts on hepatic cell line HepG2 and their mechanisms
- VernacularTitle:莪术提取物对肝癌细胞系HepG2的抗癌作用及机制研究
- Author:
Yuan TANG
;
Xiaohui LI
- Publication Type:Journal Article
- Keywords:
curcumol;
zedoary turmeric oil;
HepG2 cell;
apoptosis;
cyclooxygenase-2;
vascular endothelial growth factor
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim Curcumol and Zedoary Turmeric Oil (ZTO) were prepared to liposome, which were used to study the anti-hepatoma effect, and its mechanism that was expected to provide theoretical support for the clinic anticarcinomal use of Zedoray Rhizome Abstracts.Methods Curcumol and ZTO were prepared to liposome by mechanical diffusion and ultrasonic techniques.Proliferation of HepG2 cell in vitro was observed with curcumol and ZTO by MTT assay. Apoptosis of cells was observed by staining with Hoechst 33258/PI and laser confocal scanning microscopy (LCSM). Expression of COX-2 mRNA and VEGF mRNA of HepG2 cell was investigated by RT-PCR.Results Envelopment ratios of curcumol liposome and ZTO liposome were 86.2% and 74.5%,respectively.Curcumol and ZTO inhibited proliferation of HepG2 cell by MTT assay.Compared with control group, Curcumol and ZTO increased the apoptosis rate of HepG2 cell significantly (P
