Distribution of SHV genotypes and resistance analysis of extended-spectrum ?-lactamases-producing klebsiella pneumoniae and escherichia coli isolates in Hefei
- VernacularTitle:合肥市产超广谱?-内酰胺酶菌株的SHV型耐药基因分布和耐药性分析
- Author:
Jun CHENG
;
Yingying WANG
;
Hui LI
;
Jiabin LI
- Publication Type:Journal Article
- Keywords:
extended-spectrum ?-lactamases;
SHV type ?-lactamases;
polymerase chain reaction;
sequencing;
genotypes;
antimicrobial resistance
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the SHV genotype and analyze resistance of extended-spectrum ?-lactamases (ESBLs)-producing Klebsiella pneumoniae (Kpn) and Escherichia coli (Eco) strains isolated from clinical patients in Hefei during 1999~2000. Methods Ninety-eight strains of Klebsiella pneumoniae and Escherichia coli conserved by Anhui center for surveillance of bacterial resistance were collected from four general hospitals, including First affiliated hospital of Anhui medical university, Anhui province hospital, first people′s hospital of Hefei and the 105th hospital of PLA, from September 1999 to September 2000. The encoding genes of SHV ?-lactamases produced by ESBLs-producing isolates were amplified by PCR. The purified PCR products were cloned into pGEM-Teasy vectors and sequenced by sanger′s dideoxy chain termination composition method recommended by CLSI. Their resistances to 11 antimicrobial agents were tested by agar dilution. MICs of the antimicrobial agents against clinical isolates and the transconjugants were determined. Results Twenty-nine out of ninety-eight ESBLs-producing isolates produced SHV ?-lactamases. Of twenty-nine isolates producing SHV ?-lactamases, eight strains produced SHV-1, five strains produced SHV-11, eleven strains produced SHV-12, one strain produced SHV-18, one strain produced SHV-28, and three produced a novel SHV type SHV-89 (Accession No. DQ193536). All the strains had the same susceptible pattern, with the low susceptibility rate to piperacillin, ceftriaxone and cefotaxime. All the strains were susceptible to imipenem. Compared with clinical strains, the transconjugants showed higher resistance to many antimicrobial agents. Conclusions The rates of SHV ?-lactamases-producing klebstella. pneumoniae and Escherichia coli reach a high level in hospitals in Hefei. This is the first report on SHV-89 in China. The high resistance may be present in these clinical isolates in Hefei area.
