Establishing the method of measurement of glucose concentration in brain microdialysate by HPLC-NCSE100-ED
- VernacularTitle:建立HPLC-纳米铜网版印刷电极-电化学检测器测定透析液中葡萄糖含量的方法
- Author:
Wei WANG
;
Danqiao WANG
- Publication Type:Journal Article
- Keywords:
glucose;
microdialysis;
HPLC-nano-copper screen-printed electrode-electrochemical detector
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the rapidly detecting method of glucose concentration in brain microdialysate.Method Using high-performance liquid chromatography (HPLC) with electrochemical detector (ED) and Nano-Copper screen-printed electrode (NCSE100)-Flow injection analysis the glucose concentration in brain microdialysate were detected.Result The related coefficient (r) of glucose standard curve was 0.9995. The variation coefficient (CV) within a day was 4%~7%. The striatum extracellular glucose concentration in rats was(2.4?0.8) mmol?L-1. These results indicated that our method was reliable.Conclusion Using HPLC with ED, NCSE100, and flow injection analysis the glucose concentration in brain microdialysate were detected, which has certain applied worth.
