Influence of ERK1/2 signal-transduction pathway on differentiation of mesencephalic neural stem cells induced by bone marrow stromal cell-conditioned medium
- VernacularTitle:ERK1/2信号转导通路对骨髓基质细胞条件培养液诱导大鼠中脑神经干细胞分化作用的影响
- Author:
Ping GU
- Publication Type:Journal Article
- Keywords:
ERK1/2 signal-transduction pathway;
bone marrow stromal cells;
conditioned medium;
neural stem cells
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of the ERK1/2 signal-transduction pathway on differentiation of rat mesencephalic neural stem cells(NSCs)in presence of bone marrow stromal cell-conditioned medium(BMSCs-CM)in vitro.Methods:NSCs were cultured on poly-L-lysine(PLL)coated 35 mm dishes.The neurospheres adhered to the bottom 30 min later;then the culture medium of natural differentiation group was replaced by neurobasal medium containing 2% B27;the control group was replaced with BMSCs- CM;and the inhibition group was replaced with BMSCs-CM containing PD98059(5?mol/L).The proportions of neurons and astrocytes and their morphological difference were observed by immunocytochemistry technique 7 d later.Results:We successfully cultured NSCs from adult rat BMSCs-CM.The proportion of neurons in the natural differentiation group(15.70?10.3)% was lower than that in the control group([51.17?10.30]%,P
