Physicochemical property and in vitro antitumor activity of arsenic trioxide albumin microspheres
- VernacularTitle:三氧化二砷白蛋白微球的理化特性及抗肿瘤活性检测
- Author:
Huajiang CHEN
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
albumin microsphere;
physicochemical property
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare arsenic trioxide(As_2O_3)albumin microspheres and to analyze their physicochemical properties and in vitro antitumor activities.Methods:As_2O_3 albumin microspheres were prepared by emulsification-heat solidification.Physicochemical and the slow-release properties of the microspheres were identified by the electron microscope, thermal stabilization analysis and dynamic dialysis system in vitro.MTT method was used to evaluate the inhibitory effect of the released fluid on human osteosarcoma cell line U-2 OS cells and human lung adenocarcinoma cell line SPC-A-1 cells.Results: The mean diameter of the microspheres was(156?3.56)?m(ranging 56.4-256.7?m),with the As_2O_3 content being (55.22?11.19)% and t_(1?2)of release time being 23.34 h.The slowly released fluid showed a significant inhibitory effect on U- 2 OS and SPC-A-1 cells.Conclusion:As_2O_3 albumin microspheres prepared in the present study have satisfactory physicochemical,slow-releasing,and tumor-inhibition properties.