Effect of nitric oxide on myocardial cell injury after hypoxia in rats
- VernacularTitle:不同浓度NO对缺氧心肌细胞损害的影响
- Author:
Ming ZHANG
;
Yuesheng HUANG
;
Qiong ZHANG
- Publication Type:Journal Article
- Keywords:
hypoxia;
nitric oxide;
S-nitrosoglutathione;
myocardial function
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the effect of NO on hypoxic myocardium in rats.Methods Myocardial cells from neonatal Wistar rats aged 1-2 days cultured for 4-6 days,then pretreated with GSNO(30,200,4 000 ?mol/L)were exposed to normoxia(N)or hypoxia(H)(1%O2,5%CO2 and 94%N2)for 4 h.The NO content and LDH in the supernatant were detected.Results NO of 1.43,7.2 and 70 ?mol/L were detected respectively in the supernatant of hypoxic myocardial cells treated by GSNO(30,200 and 4 000 ?mol/L).Compared with normoxic group,LDH activity increased and myocardial cell activity decreased markedly in H+G30 and H+G4000 groups but less injury was observed in H+G200 group.Conclusion NO of certain concentration can markedly abate myocardial cell injury induced by hypoxia.
