Effect of pseudo CO_2 pneumoperitoneum on cell adhesion capability of cultured endometrium cancer cells
- VernacularTitle:模拟CO_2气腹对人子宫内膜癌细胞黏附能力的影响
- Author:
Shi CHEN
;
Zhiqing LIANG
;
Junling YAN
- Publication Type:Journal Article
- Keywords:
carbon dioxide;
endometrium cancer;
?1-integrin;
E-cadherin;
cell adhesion
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CO2 pneumoperitoneum on the cell adhesion capability of cultured endometrium cancer cells.Methods After treated in a pneumoperitoneum model for 4 h,the changes in cell growth and adhesion of the endometrium cancer were detected by MTT.The expression of ?1-integrin and E-cadherin in the cancer cells were compared with the control that were not treated with CO2,by the immunohistochemical method.Results After treated with CO2 for 4 h,the cell growth was enhanced and the cell adhesion capability increased as compared with the control group.At the same time,the expression of ?1-integrin and E-cadherin was lower in treated cells than that in untreated ones.Conclusion The adhesion capability of endometrium cancer cells in CO2 pneumoperitoneum could be enhanced by the reduction of the expression of ?1-integrin and E-cadherin,and thereby influences the metastasis of cancer.
