Effects of culture supernatant of Toxoplasma gondii on CD4~+CD25~+ T cells in mice in vitro
- VernacularTitle:弓形虫培养上清体外对CD4~+CD25~+T细胞的影响
- Author:
Gai ZHANG
;
Yiyue GE
;
Jiangping WU
;
Yong WANG
;
Wei HU
;
Mingjuan TAN
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
CD4+CD25+ T cell;
Suppression;
Apoptosis
- From:
Chinese Journal of Schistosomiasis Control
1989;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of culture supernatant of Toxoplasma gondii on CD4+CD25+ T cells in mice in vitro. MethodsCD4+CD25+ T cells were separated from spleens of C57BL/6 mice and incubated in the culture superntant of Toxoplasma gondii. The apoptosis percentage of the CD4+CD25+ T cells were detected by FACScan, and the suppression of the CD4+CD25+ T regulatory cells were examined by 3H-TdR incorporation. ResultsCompared with the CD4+CD25+ T cells incubated with RPMI-1640, (36.90?0.36)% CD4+CD25+ T cells took apoptosis after incubated with the culture supernatant of Toxoplasma gondii for 10 hours,and the Annexin-v positive rate increased by (13.60?2.15)%. Compared with RPMI-1640, the culture supernatant of Toxoplasma gondii incubating the CD4+CD25+ T regulatory cells for 5,10 hours reduced their suppressive potential on the proliferation of the CD4+CD25- T cells significantly. ConclusionsSome composition of the culture supernatant of Toxoplasma gondii might cause apoptosis of CD4+CD25+ T cells and as a result, could reduce their suppression on CD4+CD25- T cells.
