Effect of Emodin on the Apoptisis and Intracellular Ca~(2+) Concentration of Hepatic Stellate Cell-T_6
- VernacularTitle:大黄素对肝星状细胞凋亡及胞内钙离子浓度的影响
- Author:
Jianhua YU
;
Zhaosheng HUANG
- Publication Type:Journal Article
- Keywords:
Emodin;
Hepatic stellate cell;
Apoptosis;
Calcium iron
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of emodin on the apoptisis and intracellular Ca2+ concentration of Hepatic Stellate Cell-T6.Methods The immortalized rat hepatic stellate cell line HSC-T6 was commonly allocated into normal control group and treated groups with different concentrations emodin(the extreme concentration are 10?mol/ml, 20?mol/ml, 40?mol/ml respectively). The cell apoptosis rate were analyzed by flow cytometry, and intracellular Ca2+ concentration of HSC-T6 were detected by Furo-3/Am fluorescence assay and confocal laser microscopy.Results After intervention of different concentration emodin,the apoptosis rate of HSC-T6 is 4.0?0.5%, 4.3?0.6%, 6.9?1.1% respectively,and there are statistical significance compared with the control group(P
