Effects of the injectable osteoinductive material with fibrin glue as carrier on the proliferation and differentiation of marrow matrix cell of rabbit
- VernacularTitle:以纤维蛋白胶为载体的注射型骨修复材料对兔骨髓基质细胞增殖和分化的影响
- Author:
Geng CUI
;
Jie LI
;
Wei LEI
- Publication Type:Journal Article
- Keywords:
marrow stromal cells;
fibrin tissue adhesive;
cell culture;
bone morphogenetic proteins;
fibroblast growth factor,basic
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the injectable osteoinductive material with fibrin glue(FG)as carrier compounded with rhBMP-2 and bFGF on the proliferation and differentiation of marrow matrix cells(MMCs)of rabbit,in order to lay an experimental foundation for clinical application in the future.Method MMCs were isolated and cultured from bone marrow of a 3-day-old rabbit.The effects of the material were investigated in experiment group(group A:FG+bFGF+rhBMP-2),with control groups consisting of control group 1(group B:FG),control group 2(group C:FG+bFGF),control group 3(group D:FG+rhBMP-2)and single control group(group E:free of material).The observation objects included proliferation rate,adhesive rates,expression of type I collagen and alkaline phosphatase,cell growth condition in the material and the ultrastructure of rabbit MMCs with the aid of electron microscopy,histochemistry and cell culturing.The concentration of rhBMP-2 and bFGF in all groups was uniform(1?g/ml).Results The proliferation rates and the adhesive rates of rabbit MMCs in group A was significantly higher than that in group B,group D and group E,but was lower than in group C(P
