Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library
- VernacularTitle:用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白
- Author:
Ying HUANG
;
Xuefei CAI
;
Jun ZHANG
;
Ailong HUANG
- Publication Type:Journal Article
- Keywords:
T7-phage display;
hepatitis C virus core protein;
interacting proteins
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify proteins that interact with the hepatitis C virus core protein by means of T7-phage display system. Methods The hepatitis C virus core protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 human liver cDNA library. The selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Results After BLAST in all positive clones, one protein--Smad interacting protein 1 (SIP1) was found to interact with the hepatitis C virus core protein. Conclusion T7-phage display system is a convenient, rapid and effective method for screening interacting proteins.
