Expression,purification and bioactivity identification of recombinant SCR15-18 domain of human soluble complement receptor type 1
- VernacularTitle:重组人可溶性补体受体1型SCR15-18片段表达纯化及生物活性鉴定
- Author:
Bing TAN
;
Dechun ZHANG
;
Zhengqing WANG
- Publication Type:Journal Article
- Keywords:
soluble complement receptor type 1;
expression;
inclusion body;
purification;
refolding
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare highly expressed, purified and refolded SCR15-18 of human soluble complement receptor type 1 (sCR1-SCR15-18) protein. Methods The expression of recombinant pET32-sCR1-SCR15-18 in E.coli.. BL21 was induced by IPTG of different concentrations for different time period under different temperatures and the bacteria were split by sonication. The sCR1-SCR15-18 protein was purified by Ni2+-NTA resin affinity chromatography. The purified protein was refolded under different conditions. Then the bioactivity of the protein was analyzed. Results The sCR1-SCR15-18 protein of high expression, purity and bioactivity was attained. Conclusion The parameters of expression, purification and refolding of sCR1-SCR15-18 protein were optimized, which may pave a way for further studies.
