Regulation of glutamate current by orexin A on pyramidal neurons in rat prefrontal cortex
- VernacularTitle:大鼠前额叶皮层锥体细胞中Orexin A对谷氨酸介导电流的调节效应
- Author:
Chunqing ZHANG
;
Jianxia XIA
;
Penghui CHEN
;
Zhian HU
- Publication Type:Journal Article
- Keywords:
orexin;
glutamate;
wakefulness
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the modulatory effect of orexin A on glutamate receptor-mediated current in the freshly isolated pyramidal neurons from the rat prefrontal cortex (PFC). Methods Deep layer (Ⅴ-Ⅵ) prefrontal cortical pyramidal neurons from postnatal 10 to 14 day-old Wistar rats were acutely dissociated by a combination of mechanical and enzymatic method. Subsequently, the effect of orexin A on the current induced by glutamate was studied by the technique of whole cell patch clamp. Results Both orexin A and glutamate dose-dependently evoked the inward transmembrane current. The current was evoked by 1 mmol/L glutamate as a control group(100%). After treatment with 1 mmol/L orexin A for 4-10 s, 1 mmol/L glutamate induced-current was increased by (46.59?15.19)% (n=8, P
