Nervous control on the activity of migration inhibitory factor in experimental colitis
- VernacularTitle:实验性结肠炎中移动抑制因子活性的神经调节
- Author:
Ping LIN
;
Xingyu WU
;
Hui PAN
;
Shengdi HU
;
Lin MEI
- Publication Type:Journal Article
- Keywords:
colitis;
enteric nervous system;
migration inhibitory factor;
dinitrochlorobenzene;
rat;
mouse
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the expression of migration inhibitory factor (MIF) in the enteric neurons,and to explore the nervous regulation on MIF activity in experimental colitis.Methods Colitis was induced in sensitized rat and mouse by 2,4-dinitrochlorobenzene(DNCB)enema.MIF activity was measured both in the mesentery lymphocyte(by MTT)and in the enteric neurons(by immunofluorescence double staining).6-OHDA was intraperitonealy (ip) administered to mouse before DNCB treatment.Norepinephrine(NE) was added to lymphocyte culture in vitro during MIF preparation.Results The expression of MIF protein in enteric neuron was increased in DNCB-induced colitis in rat.ip 6-OHDA in colitis mouse(38～150 mg?kg-1) resulted in a further increase of MIF activity than ip vehicle in colitis mouse (P
