Prokaryotic expression and identification of human HT036 protein
- VernacularTitle:人HT036蛋白的原核表达及鉴定
- Author:
Xu PENG
;
Shunzong YUAN
;
Xiaojuan WANG
;
Chengjun GAN
;
Qinghong WANG
;
Xiwei CHEN
;
Xiaorong ZHANG
;
Xiaohong HU
;
Gaoxing LUO
;
Jun WU
- Publication Type:Journal Article
- Keywords:
HT036;
prokaryotic expression;
hypertrophic scar
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express human HT036 protein in Escherichia coli(E.coli.)and identify it.Methods The cDNA sequence obtained by PCR was cloned into the prokaryotic expression plasmid pET30a(+).The target protein was expressed in E.coli..induced by IPTG and analyzed by Western blotting.Results The interest gene was identified by restriction endonucleases digestion and DNA sequencing.The protein was highly expressed in E.coli..Conclusion We successfully expressed the HT036 protein.
