Silence the Expression of Bcl-2 Gene in Cisplatin-Resistant Cell Line Acc-3/Cddp
- VernacularTitle:重组干扰质粒对多药抗性Acc-3细胞Bcl-2基因表达作用实验研究
- Author:
Fujun ZHANG
;
Ping JI
;
Qingshu LI
;
Jinsong ZHANG
- Publication Type:Journal Article
- Keywords:
Recombined interfering plasmid;
Liposomes transfection;
Gene expression;
Fluorescence-quantitative RT-PCR
- From:
Journal of Medical Research
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interfering efficiency of RNAi technique on the expression of Bcl-2 oncogene in human oral adenoid cystic carcinoma (Acc-3/CDDP).Methods The recombined RNAi plasmids for Bcl-2 oncogene were constituted by the four successive steps-designing of Oligo DNAs, synthesis of Oligo DNAs, transfection of Oligo DNAs into pSUPER.neo+gfp vectors and selection of positive plasmids. In order to silence the expression of Bcl-2 oncogenes, the recombined RNAi plasmids were transfected into Acc-3/CDDP cells by culturing together for about 10 hours, and the interfering efficiency of RNAi for the two oncogenes was evaluated by fluorescence-quantitative RT-PCR.Results The interfering efficiencies for Bcl-2 oncogene were 0,66.20% and 0, respectively in psiB1、siB2、psiB3.Conclusions The recombined RNAi plasmids of psiB2 (CCgggAgATAgTgATgAA) for Bcl-2 oncogene can effectively silence the expression of Bcl-2 oncogenes in Acc-3/CDDP cell line.
