Effects of endotoxin on the activation of nuclear factor-kappaB in myocardium and its significance
- VernacularTitle:内毒素对心肌组织核因子-?B活化的影响及其意义
- Author:
Xiaohui DU
;
Yongming YAO
;
Rong LI
- Publication Type:Journal Article
- Keywords:
NF-kappaB;
tumor necrosis factor;
endotoxins;
myocardium
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of endotoxin on the activation of nuclear factor-kappaB in myocardium, and to explore the molecular mechanism of acute myocardial injury. Methods Forty male Wistar rats were randomly divided into normal control group (n=10), lipopolysaccharide (LPS) 1h, 2h, 6h groups (n=10 for each group). In the latter two groups, LPS was injected into the peritoneal cavity. Tissue samples from the myocardium were collected to determine NF-?B activation by electrophoretic mobility shift assay (EMSA), and the mRNA expression of TNF-? was measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) with GAPDH as internal standard. Results The activation of NF-?B (2.173?0.063) and TNF-? mRNA expression (0.292?0.031) could be detected in myocardium in very low values in normal control group rats, while both the activation and expression were up-regulated markedly after LPS challenge, and peaked 2 hours afterwards (37.793?4.785 and 1.182?0.146, respectively). They were down-regulated (17.910?3.791 and 0.901?0.128, respectively) 6 hours after LPS challenge. Compared to normal controls, both NF-?B activity and TNF-? mRNA expression were significantly elevated at various time points following LPS stimulation (all P
