Isolation and culturing of rat bone marrow mesenchymal stem cells in vitro and CFSE labeling
- VernacularTitle:大鼠间充质干细胞的体外分离培养及CFSE标记
- Author:
Xiafei FU
;
Yuanli HE
- Publication Type:Journal Article
- Keywords:
stem cells;
5,6-carboxyfluorescein diacetate succinimidyl ester;
biological markers;
cell proliferation
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05). Conclusions Highly purified MSCs can be obtained by Percoll density gradient centrifugation combined with adherent method. CFSE can obtain high labeling efficiency for a short time, and the labeled MSCs maintained the same proliferation ability as non-labeled MSCs. However, along with passage of time, the fluorescence intensity and labeling efficiency decreased.
