Inhibition of airway inflammation of asthmatic mouse by intravenous injection of T-bet recombinant adenovirus
- VernacularTitle:静脉注射T-bet重组腺病毒对小鼠过敏性气道炎症的抑制作用研究
- Author:
Jiquan CHEN
;
Qingyu XIU
- Publication Type:Journal Article
- Keywords:
asthma;
T-bet;
GATA-3;
recombinant adenovirus
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of recombinant T-bet adenovirus (AdT-bet) on airway inflammation in mice with asthma, and to explore the relevant mechanisms. Methods C57BL/6 mice were randomly allocated into 4 groups with 12 mice each. The asthmatic model was reproduced in group A, B and C by administration of ovalbumin (OVA) in aluminum hydroxide. Mice in group A, B and C received 50?l AdT-bet (108pfu) and AdLacZ and PBS, respectively, 19 days prior to OVA challenge. Bronchoalveolar lavage fluid (BALF) was collected on the 26th day after the induction of asthma for the determination of the cellular composition and concentrations of IL-4, IL-5 and IFN ?. Meanwhile, IgE content in serum was determined. The pathological changes and the expression of GATA-3 in the lungs of these mice were investigated. Results The percentage of eosinophils (EOS) and concentrations of IL-4 and IL-5 in BALF of group A were 0.6%?0.2%, 6.8?3.7pg/ml, 12.5?4.8pg/ml, respectively, which were significantly lower than those in group B (20.9%?6.8%, 92.4?23.0pg/ml, 56.5?11.8pg/ml) and group C (20.8%?6.7%, 90.4?22.8pg/ml, 57.3?12.2pg/ml, P
