Hepatocyte growth factor protects cultured rat cardiomyocytes against apoptosis induced by gamma ray irradiation
- VernacularTitle:肝细胞生长因子在大鼠心肌细胞放射损伤中的抗凋亡作用
- Author:
Shunying HU
;
Le QIU
;
Haifeng DUAN
- Publication Type:Journal Article
- Keywords:
hepatocyte growth factor;
gamma ray;
cardiomyocyte;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Hepatocyte growth factor (HGF) has been proposed to be used as an endogenous cardioprotective agent. The aim of the present study was to investigate the anti-apoptosis effect of HGF on rat cardiomyocytes after being irradiated by single dose gamma ray. Methods The study was performed using primary cell cultures of cardiomyocytes isolated from hearts of new-born rats. After being cultured for 72h in vitro, cardiomyocytes were irradiated with gamma ray in a single dose of 20Gy. In the HGF treated groups, cells were incubated with HGF in the dose of 20 and 40ng/ml 3h prior to irradiation, respectively. At 48h post-irradiation, the concentration of LDH in the supernatant of cell culture was determined. Apoptosis and cell cycle were determined with flow cytometry. Results LDH concentration in the supernatant of cell culture of irradiated cardiomyocytes was increased significantly compared to that of un-irradiated cells (P
