Determination of Saikosaponin a,d of Radix Bupleuri from Different Areas by HPLC
- VernacularTitle:高效液相法测定不同产地柴胡药材中柴胡皂苷a、d的含量
- Author:
Jing YU
;
Weiqing LIANG
- Publication Type:Journal Article
- Keywords:
radix bupleurum;
saikosaponin a,d;
HPLC
- From:
Journal of Zhejiang Chinese Medical University
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To develop a method using HPLC for determination content of saikosaponin a and saikosaponin d of Radix Bupleuri from different areas.[Method]Column:YMC-Pack ODS-A C18(250?46 mm,5?m),mobile phase:acetonitrile-water(43∶57),detected wavelength:203nm.[Results]The regression eguations of saikosaponin a and saikosaponin d were Ya =1.223?106 X - 2.249 8?105(r = 0.999 9),Yd = 2.093?106 X - 4.786 2?105 (r = 0.999 8).The average recovery rates of saikosaponin a and saikosaponin d were 98.13 %,97.99 %.[Conclusion]The HPLC method is simply and suitable to control quantitative of radix bupleurum.
