Effect of lactobacillus acidophilus on disordered intestinal flora of mice introduced by antibiotics
- VernacularTitle:嗜酸乳杆菌对抗生素诱导小鼠肠道菌群失调的作用
- Author:
Caihong XIE
;
Jing YUAN
;
Ruijun WANG
;
Hong WEI
- Publication Type:Journal Article
- Keywords:
Ceftriaxone;
Lactobacillus acidophilus;
Intestinal Flora;
Denaturing gel gradient electrophoresis
- From:Parenteral & Enteral Nutrition
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a female SPF BALB/c mouse model of antibiotic-associated intestinal microflora disturbance and analyze the effect of lactobacillus acidophilus.Methods: Female SPF BALB/c mice were treated with ceftriaxone in drinking water for five days,and followed by oral gavage of sodium chloride as natural recovery group or lactobacillus acidophilus(108CFU/mL) as therapeutic group.After seven days the intestinal microflora of the cecum content was abstracted and computed by plate counts and 16S rDNA-based PCR-DGGE method.Results: Plate counts indicated that the number of original bacterium lacticum and bacillus bifidus decreased significantly and enterococci proliferated abnormally after antibiotic treatment.After lactobacillus acidophilus treatment,the number of physiological bacteria and enterococci go back to normal levels.DGGE profile clearly indicated that the dominant bacteria changed and the bacterial diversity of intestinal microbiota was obviously decreased in the antibiotics treated group. Conclusion: Oral gavage of lactobacillus acidophilus has adjustment effect on Ceftriaxone induced intestinal microbiota disturbance.PCR-DGGE is a rapid、effective molecule method to reveal the whole microbiota status.
