The expression of DAO in gastric cancer cells and gastric mucosa cells
- VernacularTitle:右旋氨基酸氧化酶在胃癌和正常胃黏膜细胞中的表达
- Author:
Jihong LIU
;
Weixin CAO
;
Qu CAI
;
Bingya LIU
;
Zhenggang ZHU
;
Yanzhen LIN
- Publication Type:Journal Article
- Keywords:
D-amino acid oxidase;
Gastric cancer;
D-methionine
- From:
Parenteral & Enteral Nutrition
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of D-amino acid oxidase(DAO) in gastric cancer cells and gastric mucosa cells. Methods:The expression of DAO was detected in seven gastric cancer cell lines and a normal gastric mucosa cell line GES-1 by real-time quantitative PCR and in tumor tissues and normal gastric mucous tissues of 46 patients with gastric cancer by RT-PCR.The liver and kidney tissues of nude mouse acted as positive controls.Results:DAO was expressed in the liver and kidney tissues of nude mouse,and DAO expression in kidney tissue was higher than that in liver tissue.DAO was not detected in seven gastric cancer cell lines and the normal gastric mucosa cell line GES-1.Except one tumor tissue sample,DAO was not detected in other gastric cancer tissues and all normal mucosa tissues.Conclusion:DAO was not expressed in gastric cancer cells and gastric mucosa cells.The nutritional treatment of D-Met in place of L-Met could avoid the disadvantage of methionine starvation to important organs,such as liver and kidney.
