The measurement of vitamine B in the patients with Crohn's disease by HPLC
- VernacularTitle:高效液相色谱法测定克罗恩病病人的B族维生素
- Author:
Fangnan LIU
;
Xiuzhen WAN
;
Qingsong TAO
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Vitamin B;
Crohn's disease
- From:
Parenteral & Enteral Nutrition
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the difference of Vitamin B in serum in healthy adults and patiens with Crohn's disease.Methods: 24 healthy adults and 20 patients were measured for Vitamin B1,B6,B12 in serum.A HPLC-DAD method was developed to determine vitamin B extracted directly from human serum.Purospher RP-18 column was used for the gradient elution with mobile phase: 0.04 mol phosphate buffer: methanol(60∶40).Results: The results proved to be linear in measurements of VB1,VB6 and VB12 and the correlation coefficients were 0.999 827,0.985 097 and 0.999 994,respectively.The contents of Vitamin B(VB1,VB6,VB12) in Cronhn's patients were significantly lower than in healthy adults.Conclusion: The measurements of Vitamin B in human serum by HPLC-DAD method are clinically helpful to diagnosis and treatment for Crohn's disease.
