Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats
- VernacularTitle:GDNF基因活化的细胞外基质对大鼠坐骨神经缺损的修复作用
- Author:
Anlin LIANG
;
Dianming JIANG
;
Hong AN
- Publication Type:Journal Article
- Keywords:
glial cell line-derived neurotrophic factor;
extracellular matrix;
sciatic nerve injury and repair;
rat
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of glial cell line-derived neurotrophic factor(GDNF) gene-activated matrix(GAM) in bridging rat sciatic nerve defect.Methods The peripheral nerve extracellular matrix was harvested by chemical extraction.GDNF gene-activated matrix was comprised of the extracellular matrix and plasmids encoding GDNF.Ninety adult Wistar rats were randomly divided into three groups: group A(GDNF GAM conduits),group B(extracellular matrix conduits),group C (autografts).At the end of 12 weeks,rats from each group were subjected to walking tract analysis,electrophysiological and histomorphological studies.Results GDNF DNA could be retained in GAM,promoting transgene expression in the sciatic nerve,in GAM,and more importantly,in axotomized neurons in spinal cord for 12 weeks.The number of regenerated axons for the GAM grafts in the distal sciatic nerve was lower than that for autografts(2 117?294 vs 2 474?297) at 12 weeks.The sciatic nerve function index for the GAM grafts was lower than that for autografts [(30.92?2.98)% vs(26.27?2.71) %] at 12 weeks.Conclusion GDNF GAM may be a promising alternative to autografts for repair of rat sciatic nerve.
