Silencing breast cancer cell CXCR4 gene in vitro
- VernacularTitle:siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究
- Author:
Hongyuan LI
;
Guosheng REN
;
Jinxiang TAN
- Publication Type:Journal Article
- Keywords:
breast cancer;
shRNA;
CXCR4 gene
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether shRNA-CXCR4 could inhibit the CXCR4 gene expression in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells.Methods After the knockdown of CXCR4 by shRNA,the CXCR4 mRNA expression by RT-PCR and the CXCR4 protein expression by Western blotting in breast cancer cells were examined.Results The CXCR4 mRNA expression and its protein expression were decreased significantly in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells by shRNA-CXCR4(P
