Effects of adrenomedullin on the cell proliferation in renal interstitial fibrosis mice induced by unilateral ureteral obstruction
- VernacularTitle:肾上腺髓质素在肾间质纤维化实验小鼠肾脏细胞增殖中的作用
- Author:
Lin HAN
;
Zhiqiang CHEN
;
Lijian LIU
;
Qingyou XU
- Publication Type:Journal Article
- Keywords:
unilateral ureteral obstruction;
proliferating cell nuclear antigen;
adrenomedullin;
renal interstitial fibrosis
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of adrenomedullin on cell proliferation in renal interstitial fibrosis mice. Method Renal interstitial fibrosis model was induced with unilateral ureteral obstruction (UUO) and valsartan(10 mg?kg?d-1) was given by gavage. Immunohistochemistry and RT-PCR were used to detect expressions of PCNA, Col Ⅲ and TGF-?_1 in the kidney of wild type (WT) and adrenomedullin knockout (AMKO) +/-mice. Results The number of PCNA positive cells was increased in WT-UUO group compared with WT sham group and was partially reduced by Valsartan in WT-UUOV group. AMKO group showed higher expression of PCNA than WT group, and it increased more remarkably in AMKO-UUO mice but was reduced by Valsaltan in AMKO-UUOV group. Expressions of Col Ⅲ andTGF-?_1 were upregulated in AMKO-UUO mice, and this change was inhibited by Valsaltan.Conclusion Adrenomedullin play an important role in the process of cell proliferation in renal interstitial pathological changes by UUO.
