Effect of ketamine on stimulus-induced superoxide generation and intracellular calcium in human neutrophils in vitro
- VernacularTitle:氯胺酮抑制人离体中性粒细胞释放超氧阴离子的机制
- Author:
Huangwei LU
;
Wenfei TAN
;
Junke WANG
- Publication Type:Journal Article
- Keywords:
ketamine;
superoxide generation;
calcium;
p47phox;
human neutrophils
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of two racemic ketamine, S(+)-ketamine and R(-)-ketamine on stimulus-induced superoxide generation and intracellular calcium in vitro. Methods The stimulus-induced superoxide generation in human neutrophils was determined by using method of cytochrome C reduction. The intracellular calcium in human neutrophils was measured by chemiluminescence with Fura-2 loading. The phosphorylation of p47phox of NADPH oxidase in neutrophils was detected by Western blotting. Results S(+)-Ket and R(-)-Ket inhibited fMLP-induced superoxide generation in neutrophils in a concentration-dependent manner. In the case of PMA, S(+)-Ket inhibited PMA-induced superoxide generation and elevation of intracellular calcium of neutrophils in a concentration-dependent manner, whereas R(-)-Ket slightly increased PMA-induced superoxide generation and elevation of intracellular calcium of neutrophils. On the other hand S(+)-Ket inhibited the phosphorytion of p47phox of NADPH oxidase subunit,which R(-)-Ket was increased. EGTA can abolished the inhibition of S(+)-Ket on PMA-induced phosphorytion of p47phox.Conclusion S(+)-Ket inhibits the phosphorylation of p47phox of NADPH oxidase subunit and the superoxide generation in human neutrophils via PKC-calcium signal pathway.
