Construction of basic fibrobiast growth factor adenovinis vector and its expression in human umbilical vein endothelial cells
- VernacularTitle:人碱性成纤维细胞生长因子腺病毒表达载体的构建及其在人脐静脉内皮细胞中的表达
- Author:
Lingli GUO
;
Xin XING
;
Kan LIU
;
Dali HAN
- Publication Type:Journal Article
- Keywords:
basic fibroblast growth factor 2;
adenoviridae;
gene expression) umbilical veins;
endothelium, vascular
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct an adenovirus vector harboring human basic fibroblast growth factor (bFGF) cDNA and investigate the expression of bFGF in human umbilical vein endothelial cells (HUVEC) in vitro. Methods: The adenovirus expression vector Ad5-bFGF was constructed by homologous recombination technique. The best value of MOI was tested by transfecting human umbilical vein endothelial cells (HUVEC) with Ad5-GFP. Ad5-bFGF was used to transfect HUVEC at the obtained value of MOI and the expression of bFGF protein was detected by immunocytochemistry method and Western blotting. Results: The best value of MOI for adenovirus 5 to transfect HUVEC was 200 and the transfection rate was 90%. Immunocytochemistry method and Western blotting showed that bFGF was expressed in HUVEC after transfection with Ad5-bFGF and the expression was significantly higher than that in untransfected HUVEC (P
