Changes of HIF-la and VEGF expression induced by hypoxia in rats with brain injury caused by drowning
- VernacularTitle:溺水脑损伤大鼠脑组织低氧诱导因子及血管内皮生长因子表达的变化
- Author:
Yanyan MA
;
Xin LIU
- Publication Type:Journal Article
- Keywords:
drowning;
brain injuries;
hypoxia inducible factor;
vascular endothelial growth factors
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the histomorphology/pathology changes and the expression of hypoxia inducible factor-1 alpha (HIF-1?) and vascular endothelial growth factor (VEGF) in drowning rats brain, in an attempt to explore new pathways for treatment of water drowning. Methods: The animal model of freshwater drowning was established with rats. The rats were randomly divided into the following groups: control group, drowning-to-death group and 2 min-drowning group (including 5 subgroups survived for 3, 6, 12, 24. and 40 hours after being drowned in water for 2 min [n=10]). Brain indices of rats in each group were detected and the pathological changes of cerebral tissues were observed; the expression of HIF-lo and VEGF were detected immunohistochemically and the correlation between them was analyzed. The dynamic blood oxygen saturation was detected in rats survived for 40 h after 2 min-drowning at different points. Results: Brain index of the drowning group and groups surviving for 24, 40 hours after drowning were obviously higher than that of the control group (P
