Comparative study of heterogeneity of extranodal and nodal diffuse large B cell lymphoma
- VernacularTitle:淋巴结内外弥漫大B细胞淋巴瘤分化谱系及异质性的比较性研究
- Author:
Hongfang YIN
;
Ting LI
- Publication Type:Journal Article
- Keywords:
Lymphoma,B-cell;
Lymph nodes;
Microarray analysis
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Primary nodal and extranodal diffuse large B-cell lymphoma(DLBCL)were investigated for the heterogeneity of histopathology and immunophenotype,and their relation to clinical stage,comparatively.Whether E2F1 can be used as a germinal center B cell(GCB)DLBCL marker was also discussed.Methods:Classification of histopathology and immunophenotype of 98 cases were studied by immunohistochemistry in tissue microarray.Results:Histopathologic morphology presented as:centroblastic(CB,88.8%,87/98),immunoblastic(IB,5.1%,5/98),anaplastic(ALCL,3.1%,3/98),and T cell rich B cell lymphoma(TCRBCL,3.1%,3/98).Of which,31 cases were GCB DLBCL,10(20.8%,10/48)nodal,and 21 extranodal(42%,21/50,P=0.024).The rates of Stages Ⅰ/Ⅱ in nodal and extranodal area were 48.5% and 70%,respectively(P=0.015).The rate of Stage Ⅰ/Ⅱ in GCB DLBCL(74.2%)were higher than in non-GCB DLBCL(50.7%,P=0.029).The CD10 positive rates were 36.8% and 17.1% in Stages Ⅰ/Ⅱ and Ⅲ/Ⅳ,respectively,and had significant differences(P=0.033).The CD10 positive rates were 18.8% and 38% in nodal and extranodal area,respectively(P=0.035).The positive rates of E2F1 were 38.8% and 16.5% in GCB and non-GCB DLBCL,respectively,and had significant differences(P=0.016).The positive rate of E2F1 had positive relation with the expression of CD10 and Bcl-6(P
