High glucose downregulates the expression of podocalyxin protein in glomerular podocytes of mice
- VernacularTitle:高糖引起小鼠肾小球足细胞podocalyxin蛋白的表达下调
- Author:
Jia QI
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Kidney glomerulus;
Glucose
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the expression of podocalyxin protein in glomerular podocytes by long-term high glucose exposure in vitro and in vivo.Methods:Immunohistochemical staining and computer image analysis were applied to detect the expression of podocalyxin protein in glomeruli from db/db mice and Wt mice.The effects of high glucose on the expression of podocalyxin protein were analized by Western blotting.The activation of MAPKS signaling pathway(ERK,p38 and JNK)by high glucose was also examined.Results:The expressions of podocalyxin protein in db/db mice were obviously less than that in Wt mice[(0.18?0.07)vs(0.25?0.05),P
