Studies on immunization effects of single vaccine delivery system of biodegradable microspheres of recombinant glutathione-S-transferase from Schistosoma japonicum
- VernacularTitle:重组日本血吸虫谷胱甘肽-S-转移酶可生物降解微球单剂免疫效果的研究
- Author:
Xiaoniu TANG
;
Pingping ZHOU
;
Xiyin GAO
;
Xuelong WANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Glutathione-S-transferases;
PLGA microsphere;
Vaccine
- From:
Chinese Journal of Schistosomiasis Control
1989;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the dynamic changes of antibody and cytokines in the serum induced by single vaccine delivery system (SVDS) of biodegradable microspheres of recombinant glutathione-S-transferase from Schistosoma japonicum (rSjGST) in mice. Methods By using the constructed expression plasmid pET28a(+)-SjGST, rSjGST was expressed by E.coli BL21. After purified through the column of resin,the biodegradable microspheres of rSjGST were prepared with PLGA (Poly lactic-co-glycolic acid, PLGA 75/25).Six-week BALB/c female mice were immunized subcutaneously with a single vaccine, and the blood was collected from eyes on the 6th, 9th, 12th, 15 th, 18th, 21th week respectively. The dynamic levels of serum specific antibody IgG, IL-2 and IFN-? were detected. Results Compared with the control group, there was a significant difference of serum specific antibody IgG of every immune group (P
