A simplified method for rapid isolation of Schistosoma japonicum immature eggs
- VernacularTitle:简便快速分离日本血吸虫未成熟虫卵方法的研究
- Author:
Songhua ZHOU
;
Shiping WANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Immature egg;
Isolation
- From:
Chinese Journal of Schistosomiasis Control
1989;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study a simplified method for rapid isolation of the Schistosoma japonicum immature eggs for resolving the problems of the routine method which is tedious and difficult to get intact and high-purity eggs. Methods A modified method was devised on the basis of the previous experience through repetitive sievings followed by trypsin digestion and centrifugations. Results The immature eggs with fairly high purity were obtained within a short time. The isolated eggs remained intact and the rate of recovery was significantly increased. Conclusion This method has paved the way for future molecular biology research of the immature eggs of Schistosoma japonicum.