Distribution of intravenously transplanted bone mesenchymal stem cells in ischemic brain and their effects on learning and memory in rats
- VernacularTitle:静脉内移植骨髓间充质干细胞在脑缺血大鼠脑内的分布及其对学习和记忆的影响
- Author:
Yan XU
;
Jian ZHENG
;
Zhujuan ZHOU
- Publication Type:Journal Article
- Keywords:
bone mesenchymal stem cells;
intravenous transplantation;
brain ischemia;
learning and memory
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and migration of bone mesenchymal stem cells (BMSCs) in the brain after intravenous injection into brain-ischemic rats and to explore the effects on the learning and memory in rats. Methods Bone mesenchymal stem cells from adult rats were cultured in vitro. Then the cells were labeled by Hoechst 33342 and infused into the adult rats on day 3 after the ischemia/reperfusion model was established by cerebral artery occlusion. Morris water maze test was used to explore the effects on learning and memory function of the rats at 3 weeks after transplantation. Fluorescence microscope was used to identify BMSCs in rat brains at 4 weeks after transplantation. Results All indices in Morris water maze test were significantly different from those of control group at 3 weeks BMSCs transplantation(P
