The Effect of Docetaxel on Increasing Radiosensitivity of SPC-A1 Lung Cancer Cell in Vitro
- VernacularTitle:多烯紫杉醇对SPC-A1肺癌细胞放射增敏作用及其机制的实验研究
- Author:
Zhenyi REN
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Radiotherapy;
Carcinoma
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To observe the radiosensitizing effect of docetaxel on SPC-A1 lung cancer cell line and probe its radiosensitizing mechanisms in vitro. Methods After exposed to docetaxel, SPC-A1 lung cancer cells were irradiated with 137Cs ?-rays radiator at single dose of 2 to 10 Gy. For each radiation dose docetaxel-treated cultures and non-treated control cultures were irradiated simultaneously. The cell survival faction (SF) was evaluated using a standard colony-forming assay. Then the effects of docetaxel on the cell cycle and intracellular reactive oxygen species (ROS) were assayed so as to explore radiosensitizing mechanisms. Results The enhanced radiosensitivity of SPC-A1 lung cancer cells has been observed. The sensitivity enhancement ratio (SER) at 37% survival level were 1.10 and 1.36 for 24 and 48 h pretreatment of docetaxel at dose of 1.25?10-10 mol/L, while it were 1.23 and 1.43 at dose of 2.5?10-10 mol/L for 24 and 48 h pretreatment, respectively. The dose of docetaxel, which can sensitize the cells, could increase the intracellular production of ROS for nearly 2-3 times and did not result in any cell cycle redistribution. Conclusions This study demonstrated that docetaxel is synergistic with radiotherapy to lung cancer cell in vitro. ROS may play a substantial role in the process docetaxel-induced radiosensitization.
