The Cultured Human Conjunctival Epithelium Combined with Amniotic Membrane Transplantation for Treatment of Conjunctival Defect
- VernacularTitle:兔眼结膜上皮细胞体外培养治疗结膜缺损实验研究
- Author:
Jianguang ZHONG
;
Zhong LU
;
Huicheng ZHANG
- Publication Type:Journal Article
- Keywords:
Rabbits;
Cell culture techniques;
Conjunctiva
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of the cultured human conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect. Methods 20 eyes of 10 rabbits were divided into study group (10 right eyes ) and contoal group (10 left eyes ). The conjunctival epithelium got from conjunctiva of fornix of study group about 1mm?1mm was explanted on human amniotic membrane and was cultured in vitro by tissue inoculation 2 weeks before operation. The bulbar conjunctivals of 20 eyes were excised. The conjunctival defect was covered by the graft in the study group and uncovered in the control group. The growth features of the grafts were observed. Results The conjunctival epithelium explanted on human amniotic membrane connected each other into membrane in 6 days, and multi-layer cells were observed. In the study group, all grafts were inosculated with and were similar to normal conjunctival in 2 weeks. There was not immune rejection occurred. In the control group, the conjunctival defect was recovered by scar. Conclusion It is effective of the cultured conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect.
