Biological dose estimation by cytokinesis-block micronucleus assay on the patients exposed to accidental radiation
- VernacularTitle:胞浆分裂阻滞微核法对山东“10?21”辐射事故病人受照射剂量的估算
- Author:
Bo YAO
;
Benrong JIANG
;
Shuqiong JIA
- Publication Type:Journal Article
- Keywords:
radiation injuries;
micronuclei;
biological dose estimation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A biological dose estimation by cytokinesis-block micronucleus(CBMN)assay was performed on 2 patients exposed to accidental radiation.Method Micronucleus assay and dose estimation by CBMN in peripheral blood and bone marrow were performed in 2 patients(A and B)suffering from radiation sickness.Results No binucleated lymphocytes were observed in peripheral blood of 2 patients.The results of bone marrow examination showed that only one binucleated cell was discovered in patient A.The rough estimation of radiation dose of A was above 20Gy according to the degree of reduction of binucleated cell in our previous study.The micronucleus frequency was 2.42 per cell and the dose estimation was 8.7(8.0-9.4)Gy for patient B.These results were approximately in accordance with that of dose estimations by physical method,chromosome aberration analysis,ESR method,and clinical manifestations.Conclusion MN assay is simple,quick and accurate.It is a reliable biodosimetry in addition to chromosome aberration analysis.
