Dynamic observations on the cytogenetics of the exposed patients in Shandong “10?21” radiation accident
- VernacularTitle:山东“10?21”辐射事故病人细胞遗传学动态观察
- Author:
Bo YAO
;
Benrong JIANG
;
Shuqiong JIA
- Publication Type:Journal Article
- Keywords:
radiation injuries;
hematopoietic stem cell transplantation;
cytogenetic analysis
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the engraftment state after allogeneic peripheral blood stem cell transplantation(Allo-PBSCT)in 2 exposed patients in Shandong “10?21” radiation accident.Methods The dynamic cytogenetic analysis was performed by chromosome aberration(CA)analysis,R-band karyotyping analysis and micronucleus(MN)assay of peripheral blood and/or bone marrow samples of 2 patients(A and B).Results The results showed that the transplantation was successful(complete chimerism),and the engraftment state was stable.No difference was observed between the results of cytogenetic analysis from peripheral blood samples and bone marrow samples.These results were in fairly good accordance with those results by short tandem repeats-polymerase chain reaction(STR-PCR)and clinical manifestations.Conclusions The results suggested that the dynamic observations of dicentric and ring aberrations and MN can be used as an indication to estimate the engraftment state after Allo-PBSCT,but only for the patient with acute radiation sickness.Moreover,the cytogenetic analysis performed on bone marrow samples is better than that on peripheral blood samples when the lymphocytes were reduced sharply in peripheral blood.
