Induced expression of indoleamine 2,3-dioxygenase in epithelial cells of human proximal renal tubule
- VernacularTitle:人近端肾小管上皮细胞诱导表达吲哚胺2,3-加双氧酶的实验研究
- Author:
Weiping HOU
;
Fahuan YUAN
- Publication Type:Journal Article
- Keywords:
indoleamine 2,3-dioxygenase;
interferon type Ⅱ;
human proximal tubular epithelial cells
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the induced expression of indoleamine 2,3-dioxygenase in epithelial cells of cultured human proximal renal tubule under the stimulation of interferon-?(IFN-?)in vitro.Methods The expression of IDOmRNA in epithelial cells of human proximal renal tubule under normal and different dosage or time of IFN-? stimulation were assayed by semi-quantitative RT-PCR,the expression of IDO protein under normal and 2000U/ml IFN-? stimulation for 24h were assayed by immunocytochemistry,and the activity of IDO under different dosage of IFN-? stimulation was assessed by the special kynurenine metabolizable ratio in culture medium detected by high performance liquid chromatography(HPLC).Results No expression of IDO mRNA or IDO was found in the epithelial cells of human proximal renal tubule under normal culture conditions.IDO mRNA expression increased significantly after IFN-? stimulation in a dose dependent manner compared with that of control group(P
