In vitro photodynamic therapy of human nasopharyngeal carcinoma cell line CNE2 using 5-aminolevulinic acid
- VernacularTitle:5-氨基乙酰丙酸光动力杀伤人鼻咽癌细胞株的实验研究
- Author:
Xiaomin YANG
;
Rongcheng LUO
;
Libo LI
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
5-aminolevulinic acid;
photochemotherapy
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of using 5-aminolevulinic acid(5-ALA)induced protoporphyrin IX(PpIX)for photodynamic therapy(PDT)of nasopharyngeal carcinoma(NPC)cell line CNE2 cultured in vitro.Methods CNE2 cells cultured were incubated in a medium containing various concentrations of 5-ALA(0.01,0.05,0.1,0.25,0.5,1.0,2.0,4.0mmol/L)for 6 hours,then illuminated with different light doses(20,10,5,2J/cm2)using a semiconductor laser at 630nm.After 6,12,24 hours incubation with 5-ALA-PDT,the survival rates of CNE2 cells were analyzed by MTT assay.Results 5-ALA-PDT may effectively kill the CNE2 cells.The killing degree was positively correlated with the incubation time after irradiation,concentration of 5-ALA and the dosage of radiation(P
