Effects of soy bean isoflavone on the expression of VEGF and NOS subtype mRNA in ovaries during perimenopause period in rat
- VernacularTitle:大豆异黄酮对围绝经期大鼠卵巢VEGF和NOS亚型mRNA表达的影响
- Author:
Haixia WEN
;
Guoyi LIU
;
Wei ZHAO
- Publication Type:Journal Article
- Keywords:
soy isoflavones;
perimenopause;
ovary;
vascular endothelial growth factors;
nitric-oxide synthase
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of soy bean isoflavone(SI)on the expression of VEGF,nitric oxide synthase(NOS)subtype,endothelial NOS(eNOS)and inducible NOS(iNOS)mRNA in ovaries during perimenopause period in rats.Methods A rat model of perimenopause was reproduced by enforced ageing.42 female Wistar rats of 12 months old were randomly divided into five groups:aging model group,low,middle,high doses of SI groups,and nilestriol(NI)group.10 female rats of 3 months old served as control group.The animals in low(50mg/kg),middle(158mg/kg)and high(500mg/kg)dosage of SI groups,and in NI group(0.158mg/kg)were gavaged with corresponding drugs for 8 weeks.The expressions of VEGF and NOS mRNA in ovaries were detected by RT-PCR.The total NOS activity in serum and ovaries was detected by chromatometry.Results Compared with control group,the expressions of VEGF mRNA in ovaries of perimenopause rats increased significantly(P
