Effect of XueBiJing injection on the expression of high mobility group box-1 protein in lung of rats with burn injury
- VernacularTitle:血必净注射液对烫伤大鼠肺组织高迁移率族蛋白B1表达的影响及意义
- Author:
Qiang WANG
;
Yongming YAO
;
Wenjiang WANG
- Publication Type:Journal Article
- Keywords:
burns;
XueBiJing;
HMGB1 protein;
acute lung injury
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of XueBiJing injection(a concoction of Chinese herbs)on the expression of high mobility group box-1 protein(HMGB1)in the lung with acute lung injury of rats following delayed resuscitation after burn injury.Methods 78 male rats were randomly divided into sham scald group(n=18),scald group(n=30)and XueBiJing treatment group(n=30).In the scald group and XueBiJing treatment group,4ml/kg normal saline or XueBiJing was intravenously injected 2 hours after scald injury.In the latter two groups,rats were subjected to 30% TBSA full-thickness scald injury,and fluid resuscitation was delayed.The animals were sacrificed at 8h,24h and 72h after injury,respectively.Lung tissue samples were collected to determine the expression of HMGB1 mRNA and protein,and the activity of pulmonary myeloperoxidase(MPO).HMGB1 mRNA level was semi-quantitatively measured by RT-PCR using GAPDH as an internal standard,and protein expression of HMGB1 was determined by Western blot.Results Compared with sham scald controls,both mRNA and protein expressions of HMGB1 were significantly enhanced in the lung at 8-72h after scald injury(P
